6JM5

Crystal structure of TBC1D23 C terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97921
Spacegroup name	C 1 2 1
Unit cell lengths	130.822, 48.950, 47.439
Unit cell angles	90.00, 109.64, 90.00

Refinement procedure

Resolution	45.530 - 1.600
R-factor	0.159
Rwork	0.157
R-free	0.20730
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.257
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.056	0.035
Rmeas	0.061	0.038
Rpim	0.025	0.015	0.641
Total number of observations	251366
Number of reflections	37552	1934	1886
<I/σ(I)>	7.4
Completeness [%]	99.6	98.3	99.7
Redundancy	6.7	6.8	6.7
CC(1/2)		0.995	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M MES pH6.5, 0.5 M Ammonium nitrate, 25% polyethylene glycol 400