6JLZ
P-eIF2a - eIF2B complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 155.578, 207.712, 155.744 |
| Unit cell angles | 90.00, 96.96, 90.00 |
Refinement procedure
| Resolution | 49.313 - 3.350 |
| R-factor | 0.2211 |
| Rwork | 0.221 |
| R-free | 0.25260 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.633 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.550 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 140658 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium acetate, sodium citrate, PEG 4000, glycerol |
