6JLE

Crystal structure of MORN4/Myo3a complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.989
Spacegroup name	P 43
Unit cell lengths	73.735, 73.735, 48.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.975 - 1.550
R-factor	0.1621
Rwork	0.161
R-free	0.18600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.070	0.034	1.138
Rmeas	0.075	0.036	1.226
Rpim	0.028	0.014	0.455
Number of reflections	37810	1950	1914
<I/σ(I)>	9.7
Completeness [%]	99.9	98.9	100
Redundancy	7.3	7.3	7.2
CC(1/2)		0.999	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	289	0.5 M ammonium sulfate, 1.0 M lithium sulfate, 0.1 M sodium citrate tribasic pH 5.6