6JLD

Crystal structure of a human ependymin related protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97935
Spacegroup name	P 43
Unit cell lengths	55.858, 55.858, 273.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.710 - 2.000
R-factor	0.20139
Rwork	0.199
R-free	0.23497
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	56296
<I/σ(I)>	37.7
Completeness [%]	99.8
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350 3% Methanol