6JL3

Crystal Structure of the UBL domain of Plasmodium Falciparum Dsk2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	70.927, 70.927, 28.122
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.460 - 1.303
R-factor	0.1569
Rwork	0.156
R-free	0.16770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwf
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.463	1.350
High resolution limit [Å]	1.303	1.303
Rmerge	0.044	0.630
Rmeas	0.047	0.630
Rpim	0.016	0.246
Number of reflections	18008	797
<I/σ(I)>	24.47
Completeness [%]	99.5	95.89
Redundancy	8.1	7.3
CC(1/2)	1.000	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Bis-Tris pH 6.5 , 2M ammonium sulfate