6JL2

Crystal structure of VvPlpA G389N from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	167.946, 64.551, 160.649
Unit cell angles	90.00, 116.55, 90.00

Refinement procedure

Resolution	46.000 - 2.300
R-factor	0.175
Rwork	0.174
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jkz
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.680	2.337
High resolution limit [Å]	2.298	2.298
Rmeas	0.059	0.229
Rpim	0.023	0.095
Number of reflections	63445	2593
<I/σ(I)>	21.8	5.9
Completeness [%]	91.9	76.2
Redundancy	6.6
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4 mg/ml in 10 mM HEPES, 150 mM NaCl, pH 7.5 was mixed with the reservoir solution (200 mM potassium sodium tartrate tetrahydrate, 180 mM NDSB-201, 14% polyethylene glycol 1500, and 5% glycerol)