6JL0

Crystal structure of VvPlpA from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9163
Spacegroup name	P 31 2 1
Unit cell lengths	53.865, 53.865, 232.287
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.735 - 2.073
R-factor	0.1926
Rwork	0.191
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	our own model
RMSD bond length	0.014
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.736	2.109
High resolution limit [Å]	2.073	2.073
Rmeas	0.082	0.684
Rpim	0.019	0.185
Number of reflections	24564	998
<I/σ(I)>	20.9	2.8
Completeness [%]	99.1	86.5
Redundancy	18.2	12
CC(1/2)	0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5 mg/ml protein in 10 mM HEPES, 150 mM NaBr, pH 7.5, was mixed with equal volume reservoir solution (200 mM potassium sodium tartrate tetrahydrate, 180 mM NDSB-201, 12% polyethylene glycol 1500, and 10% glycerine)