6JKX

Crystal structure of peptidyl-tRNA hydrolase with multiple sodium and chloride ions at 1.08 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.97199
Spacegroup name	P 21 21 21
Unit cell lengths	34.151, 66.264, 76.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.030 - 1.080
R-factor	0.143
Rwork	0.142
R-free	0.17762
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y9a
RMSD bond length	0.018
RMSD bond angle	1.896
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.030	1.160
High resolution limit [Å]	1.080	1.080
Rmerge	0.169	1.497
Rmeas	0.178	1.588
Rpim	0.057	0.517
Number of reflections	71837	2990
<I/σ(I)>	6.9
Completeness [%]	98.6	67.3
Redundancy	7.9
CC(1/2)	0.990	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	12% PEG 1500, 0.1M HEPES PH 7.5