6JKX
Crystal structure of peptidyl-tRNA hydrolase with multiple sodium and chloride ions at 1.08 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.151, 66.264, 76.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.030 - 1.080 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.17762 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y9a |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.896 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.030 | 1.160 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.169 | 1.497 |
| Rmeas | 0.178 | 1.588 |
| Rpim | 0.057 | 0.517 |
| Number of reflections | 71837 | 2990 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 98.6 | 67.3 |
| Redundancy | 7.9 | |
| CC(1/2) | 0.990 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 12% PEG 1500, 0.1M HEPES PH 7.5 |
