6JKV
PppA, a key regulatory component of T6SS in Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-04 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.541, 80.253, 59.084 |
| Unit cell angles | 90.00, 111.81, 90.00 |
Refinement procedure
| Resolution | 24.080 - 2.100 |
| R-factor | 0.23529 |
| Rwork | 0.233 |
| R-free | 0.28332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1txo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.140 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.280 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.119 | |
| Number of reflections | 25293 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 98.6 | |
| Redundancy | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG 8000, Calcium acetate hydrate, HEPES |
