6JK0

Crystal Structure of YAP1 and Dendrin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	94.606, 94.606, 174.397
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.059 - 3.100
R-factor	0.216
Rwork	0.213
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rex
RMSD bond length	0.018
RMSD bond angle	1.265
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.225	1.000
Number of reflections	8993	444
<I/σ(I)>	15.3	2
Completeness [%]	99.9	100
Redundancy	34.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	25-30% w/v PEG400, 200mM CaCl2, 100mM HEPS (pH 7.5)