6JJZ

Crystal Structure of MAGI2 and Dendrin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	46.899, 58.582, 86.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.917 - 1.650
R-factor	0.2058
Rwork	0.204
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ysd
RMSD bond length	0.006
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.047	0.856
Number of reflections	29381	1465
<I/σ(I)>	44	2.5
Completeness [%]	99.8	99.9
Redundancy	6.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	289	2.0-2.5M ammonium sulfate, 100mM sodium acetate (pH 4.6)