6JJZ
Crystal Structure of MAGI2 and Dendrin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.899, 58.582, 86.298 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.917 - 1.650 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ysd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.003 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.047 | 0.856 |
Number of reflections | 29381 | 1465 |
<I/σ(I)> | 44 | 2.5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.9 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 289 | 2.0-2.5M ammonium sulfate, 100mM sodium acetate (pH 4.6) |