6JJW
Crystal Structure of KIBRA and PTPN14 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | H 3 2 |
Unit cell lengths | 107.630, 107.630, 107.832 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.944 - 2.400 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j68 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.121 | 0.878 |
Number of reflections | 9608 | 397 |
<I/σ(I)> | 18.2 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.9 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 700mM magnesium formate, 100mM Bis-Tris Propane (pH 7.0) |