6JJK

Crystal structure of the DegP dodecamer with a modulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	214.920, 122.427, 140.242
Unit cell angles	90.00, 117.72, 90.00

Refinement procedure

Resolution	35.479 - 3.600
R-factor	0.2027
Rwork	0.199
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3otp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.660
High resolution limit [Å]	3.600	9.750	3.600
Rmerge	0.102	0.058	0.527
Rmeas	0.110	0.064	0.566
Rpim	0.042	0.026	0.206
Total number of observations	270801
Number of reflections	37293	1879	1815
<I/σ(I)>	11.2
Completeness [%]	99.9	97.2	100
Redundancy	7.3	5.9	7.5
CC(1/2)		0.996	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	10% PEG3350 and 0.1 M tacsimate pH 3.4