6JJJ
Trimeric structure of Kupffer cell C-type lectin receptor Clec4f
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.150, 75.190, 61.200 |
| Unit cell angles | 90.01, 90.04, 120.06 |
Refinement procedure
| Resolution | 32.559 - 2.792 |
| R-factor | 0.2544 |
| Rwork | 0.250 |
| R-free | 0.29250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kqg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.690 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.800 |
| Number of reflections | 27730 | |
| <I/σ(I)> | 7.93 | |
| Completeness [%] | 93.8 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Potassium thiocyanate, 30% Polyethylene glycol monomethyl ether 2000 |
