6JJI
Crystal structure of a two-quartet RNA parallel G-quadruplex complexed with the porphyrin TMPyP4 (1:1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97900 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 23.509, 29.244, 57.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.042 - 3.100 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jjh |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.042 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.108 | 0.403 |
Number of reflections | 825 | 79 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 96.6 | |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | Potassium cacodylate, Sodium cacodylate, Potassium chloride, MPD |