6JJ9

Crystal structure of OsHXK6-Glc-ATP-Mg2+ complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	95
Detector technology	CCD
Collection date	2018-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	131.120, 131.120, 186.192
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.070 - 3.000
R-factor	0.2084
Rwork	0.206
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jj7
RMSD bond length	0.003
RMSD bond angle	0.710
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.070	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.024	0.079
Number of reflections	37637	3702
<I/σ(I)>	19.94	8.15
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2 mM D-glucose, 3 mM magnesium chloride, 2 mM ATP, 0.1 M MES 6.5, 0.05 M cesium chloride, 30% v/v JeffamineM-600.