6JJ1
Crystal structure of peptidyl-tRNA hydrolase from Acinetobacter baumannii at 0.97 A resolution with disordered five N-terminal residues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.986, 66.131, 76.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.080 - 0.970 |
| R-factor | 0.13418 |
| Rwork | 0.134 |
| R-free | 0.16705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y9a |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.006 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.080 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rpim | 0.028 | 0.629 |
| Number of reflections | 94081 | 3426 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 92.0 | |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 12% PEG 1500, 0.1M HEPES, pH 7.5, 20% Glycerol |
