6JIR
Crystal structure of C. crescentus beta sliding clamp with PEG bound to putative beta-motif tethering region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.724, 123.527, 85.975 |
| Unit cell angles | 90.00, 120.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| Rwork | 0.171 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tr8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.069 | 0.500 |
| Rpim | 0.027 | |
| Number of reflections | 74116 | 3421 |
| <I/σ(I)> | 27.9 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 25 % PEG1000, 0.2 M NaCl and 0.1 M Na/K phosphate |
