6JIA
Marine bacterial laminarinase mutant E135A complex with laminaritetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.863, 95.456, 72.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.880 - 1.900 |
| R-factor | 0.1521 |
| Rwork | 0.150 |
| R-free | 0.18670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4crq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.460 |
| Number of reflections | 18576 | 1147 |
| <I/σ(I)> | 17.5 | 3.5 |
| Completeness [%] | 99.8 | 97.4 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% (v/v) ethonal, 28% PEG20000 100 mM MES pH7.0 |
