6JHX
Crystal structure of alginate-binding protein AlgQ2 without calcium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.873, 60.824, 87.228 |
| Unit cell angles | 80.37, 89.81, 88.17 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.21324 |
| Rwork | 0.210 |
| R-free | 0.27081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j1n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.145 | 0.362 |
| Number of reflections | 47003 | 2305 |
| <I/σ(I)> | 63.6 | |
| Completeness [%] | 97.8 | 97.9 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium formic acid 0.1 M 1,3-bis tris[(hydroxymethyl)methylamino]propane (pH 8.5) 20% polyethylene glycol 3350 |
