6JHV

Structure of anti-CRISPR AcrIIC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-07-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97940
Spacegroup name	P 21 21 2
Unit cell lengths	74.208, 47.919, 71.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.707 - 2.321
R-factor	0.1956
Rwork	0.190
R-free	0.24150
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.864
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.320	2.320
Rmerge	0.110	0.429
Number of reflections	11533	553
<I/σ(I)>	38.8
Completeness [%]	99.7
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20 mM HEPES-NaOH, pH 7.4, 200 mM NaCl, 5% (v/v) glycerol, 2 mM BME