6JHK

Crystal Structure of Bacillus subtilis RsbS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	I 2 3
Unit cell lengths	179.201, 179.201, 179.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.867 - 3.101
R-factor	0.2131
Rwork	0.210
R-free	0.24050
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.717
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_2313)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.072	0.546
Number of reflections	17310	851
<I/σ(I)>	38.9	5.2
Completeness [%]	98.9	100
Redundancy	5.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6	295	MPD, LiSO4