6JH9
Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. Lys18Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95365 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.350, 56.368, 38.478 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 48.738 - 1.739 |
| R-factor | 0.1846 |
| Rwork | 0.184 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ypu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.649 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 1.770 |
| High resolution limit [Å] | 1.739 | 1.739 |
| Rmerge | 0.078 | 0.666 |
| Rmeas | 0.093 | 0.789 |
| Rpim | 0.050 | 0.419 |
| Number of reflections | 42896 | 2252 |
| <I/σ(I)> | 8 | 1.4 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298.15 | 0.1M sodium acetate trihydrate, pH 4.8, 8% PEG 4000 |
