6JH8

Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	C 1 2 1
Unit cell lengths	195.439, 56.073, 38.432
Unit cell angles	90.00, 93.40, 90.00

Refinement procedure

Resolution	42.467 - 2.149
R-factor	0.1773
Rwork	0.175
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ypu
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.470	2.210
High resolution limit [Å]	2.149	2.149
Rmerge	0.124
Rmeas	0.145
Rpim	0.075	0.524
Number of reflections	22798	1810
<I/σ(I)>	7.3
Completeness [%]	99.2	96.7
Redundancy	3.7	3.7
CC(1/2)	0.995	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298.15	0.1M sodium acetate trihydrate, pH 4.6, 10% PEG 4000