6JH0

Crystal structure of cISG15/NS1B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	77.298, 77.298, 217.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.419 - 2.403
R-factor	0.21
Rwork	0.207
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.045
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.419	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	26531
<I/σ(I)>	20.7
Completeness [%]	100.0
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M magnesium chloride, 0.1 M MES, pH 6.7, 16 %(w/v) PEG 6000