6JGY
Crystal structure of LASV-GP2 in a post fusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 58.713, 58.713, 286.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.037 - 3.389 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5omi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.214 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.037 | 3.480 |
| High resolution limit [Å] | 3.389 | 3.400 |
| Number of reflections | 2839 | |
| <I/σ(I)> | 19.15 | |
| Completeness [%] | 98.1 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.17 M Ammonium Acetate, 0.085 M Sodium Citrate:HCl, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) Glycerol |
