6JGX
Crystal structure of the transcriptional regulator CadR from P. putida in complex with Cadmium(II) and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 131.490, 131.490, 85.118 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.710 |
| R-factor | 0.21051 |
| Rwork | 0.209 |
| R-free | 0.23347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jgv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.359 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.560 | |
| Number of reflections | 22742 | 2213 |
| <I/σ(I)> | 26.2 | 2.2 |
| Completeness [%] | 98.9 | 96.9 |
| Redundancy | 6.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 289 | PEG 3350, potassium chloride, magnesium chloride, sodium cacodylate |
