6JGW
Crystal structure of the transcriptional regulator CadR from P. putida in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.967, 54.541, 86.259 |
| Unit cell angles | 90.00, 100.37, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.20654 |
| Rwork | 0.204 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jgv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.510 | |
| Number of reflections | 15413 | 1527 |
| <I/σ(I)> | 15.9 | 3.9 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 5.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | MPEG 2000, potassium thiocyanate |
