6JEH

Crystal structure of calcium free human gelsolin amyloid mutant D187Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	105
Detector technology	CCD
Collection date	2014-10-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	169.232, 169.232, 151.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.944 - 2.950
R-factor	0.1962
Rwork	0.193
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ffn
RMSD bond length	0.011
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.944	3.050
High resolution limit [Å]	2.950	2.950
Number of reflections	46032
<I/σ(I)>	1.87
Completeness [%]	98.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5