6JEG
Crystal structure of calcium free human gelsolin amyloid mutant G167R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2014-10-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 172.274, 172.274, 150.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.780 - 2.975 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.23720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffn |
RMSD bond length | 0.005 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.780 | 3.080 |
High resolution limit [Å] | 2.970 | 2.970 |
Number of reflections | 45030 | |
<I/σ(I)> | 10 | |
Completeness [%] | 96.4 | |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl, pH8.5 |