6JEF
Crystal structure of apo-R168C/L169C-Fr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-12-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.606, 181.606, 181.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.710 - 1.580 |
| R-factor | 0.16779 |
| Rwork | 0.167 |
| R-free | 0.18186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.010 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.076 | 0.246 |
| Number of reflections | 35456 | 1746 |
| <I/σ(I)> | 61.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Cadmium sulphate |
