6JEC
Structure of a triple-helix region of human collagen type II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-01 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 19.360, 22.990, 39.909 |
| Unit cell angles | 102.76, 92.14, 113.97 |
Refinement procedure
| Resolution | 20.294 - 2.049 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.294 | 2.090 |
| High resolution limit [Å] | 2.049 | 2.050 |
| Number of reflections | 3550 | |
| <I/σ(I)> | 31.6 | |
| Completeness [%] | 92.8 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M Sodium Acetate 20%(w/v) PEG 3350 |
