6JDY
Ligand complex structure of GH10 family xylanase XynAF1, soaking for 120 minutes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.330, 57.106, 64.734 |
| Unit cell angles | 74.19, 80.96, 68.87 |
Refinement procedure
| Resolution | 62.150 - 1.500 |
| R-factor | 0.1229 |
| Rwork | 0.121 |
| R-free | 0.15070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.150 | 1.555 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.091 |
| Number of reflections | 83792 | |
| <I/σ(I)> | 18.79 | 12.64 |
| Completeness [%] | 92.2 | 92.86 |
| Redundancy | 3.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES pH6.5, 25%(w/v) PEG 6000 |
