6JDU

Crystal structure of PRRSV nsp10 (helicase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-07-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	P 43 21 2
Unit cell lengths	92.914, 92.914, 147.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.810
R-factor	0.22556
Rwork	0.225
R-free	0.23827
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jdr
RMSD bond length	0.007
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.119	0.703
Rmeas	0.124	0.741
Rpim	0.035	0.230
Number of reflections	16581	649
<I/σ(I)>	19.9	2.4
Completeness [%]	97.7	81.9
Redundancy	11.8	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.03	277	PEG 400, Na HEPES, calcium chloride