6JDU
Crystal structure of PRRSV nsp10 (helicase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.914, 92.914, 147.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.810 |
| R-factor | 0.22556 |
| Rwork | 0.225 |
| R-free | 0.23827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jdr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.119 | 0.703 |
| Rmeas | 0.124 | 0.741 |
| Rpim | 0.035 | 0.230 |
| Number of reflections | 16581 | 649 |
| <I/σ(I)> | 19.9 | 2.4 |
| Completeness [%] | 97.7 | 81.9 |
| Redundancy | 11.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.03 | 277 | PEG 400, Na HEPES, calcium chloride |
