6JDR
Crystal structure of methylated PRRSV nsp10 (helicase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-07-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 93.158, 93.158, 148.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.23731 |
Rwork | 0.235 |
R-free | 0.27210 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.897 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.099 | |
Number of reflections | 23333 | 1128 |
<I/σ(I)> | 31.1 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.2 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PEG 400, Na HEPES, calcium chloride |