6JDG

Complexed crystal structure of PaSSB with ssDNA dT20 at 2.39 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	298
Detector technology	CCD
Collection date	2017-11-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.975
Spacegroup name	P 31
Unit cell lengths	60.042, 60.042, 129.642
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.247 - 2.388
R-factor	0.2157
Rwork	0.213
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yuo
RMSD bond length	0.009
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.480
High resolution limit [Å]	2.387	2.387
Rmerge	0.049	0.463
Number of reflections	20581	2089
<I/σ(I)>	20.28	2.41
Completeness [%]	99.0	99.8
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	10% PEG 8000, 200mM Magnesium Acetate