6JDD

Crystal structure of the cypemycin decarboxylase CypD.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	F 41 3 2
Unit cell lengths	229.456, 229.456, 229.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.200 - 2.600
R-factor	0.17469
Rwork	0.172
R-free	0.21930
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	2.001
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.182	0.844
Rpim	0.044	0.196
Number of reflections	16495	794
<I/σ(I)>	26.8	3.58
Completeness [%]	100.0	100
Redundancy	18.7	19.4
CC(1/2)		0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM NaCl, 0.1 M Imidazole, 10 mM Tris, pH 8.0