6JD6

An ankyrin-repeat protein complex guides cargos from inner envelope to thylakoid Tat pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	I 41 3 2
Unit cell lengths	216.816, 216.816, 216.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.482 - 2.200
R-factor	0.167
Rwork	0.166
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb5
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.076	0.031
Rmeas	0.080	0.032
Rpim	0.026	0.010	0.354
Number of reflections	44172	2403	2164
<I/σ(I)>	5.5
Completeness [%]	100.0	99.5	100
Redundancy	9.8	9.2	9.7
CC(1/2)		0.999	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	100mM Hepes pH 7.0, 0.2mM (NH4)2SO4, 25% PEG 3350