6JD4
ATPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.220, 77.314, 106.011 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.723 - 2.100 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.24640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nh0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.715 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.466 | 43.723 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.050 | 0.331 | |
Rmeas | 0.140 | 0.052 | 0.346 |
Rpim | 0.039 | 0.015 | 0.098 |
Total number of observations | 388582 | 12803 | 55736 |
Number of reflections | 30107 | 1068 | 4313 |
<I/σ(I)> | 17.9 | 31 | 9.6 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 12.9 | 12 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.49 M sodium phosphate monobasic monohydrate, 0.91 M potassium phosphate dibasic, pH 6.9 |