6JCN
Yeast dehydrodolichyl diphosphate synthase complex subunit NUS1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.328, 82.671, 131.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.837 - 1.998 |
| R-factor | 0.1537 |
| Rwork | 0.152 |
| R-free | 0.18020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hxq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.115 | 0.985 |
| Rpim | 0.027 | 0.279 |
| Number of reflections | 42913 | 4133 |
| <I/σ(I)> | 26.3 | 1.7 |
| Completeness [%] | 99.6 | 97.04 |
| Redundancy | 18.8 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M (NH4)2SO4, 0.1M Sodium Cacodylate pH 6.5, 20% PEG 8000 |
