6JCB
Crystal structure of aminotransferase CrmG from Actinoalloteichus sp. WH1-2216-6 in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.130, 114.830, 156.170 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.850 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dds |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.532 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.870 | 38.870 | 2.940 |
| High resolution limit [Å] | 2.850 | 11.400 | 2.850 |
| Rmerge | 0.117 | 0.048 | 0.453 |
| Rmeas | 0.146 | 0.061 | 0.563 |
| Rpim | 0.086 | 0.037 | 0.329 |
| Total number of observations | 117939 | 1799 | 11372 |
| Number of reflections | 47035 | 726 | 4432 |
| <I/σ(I)> | 6.1 | 14 | 2.1 |
| Completeness [%] | 93.5 | 88.8 | 95.9 |
| Redundancy | 2.5 | 2.5 | 2.6 |
| CC(1/2) | 0.981 | 0.993 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2 M Sodium acetate, 0.1 M TRIS pH 8.5, 32%PEG 3350, 2% glycerol |
