6JBX
Crystal structure of Streptococcus pneumoniae FabT in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.022, 60.868, 74.086 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 35.960 - 2.200 |
| R-factor | 0.18922 |
| Rwork | 0.188 |
| R-free | 0.22070 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.540 |
| Number of reflections | 31594 | 3169 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 6.2 | 6 |
| CC(1/2) | 0.857 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1 M sodium citrate pH 5.5, 0.2 M Sodium acetate trihydrate, 5% PEG 4000 |
