6JBM

Crystal structure of the TRIM14 PRYSPRY domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	48.326, 104.020, 93.381
Unit cell angles	90.00, 91.49, 90.00

Refinement procedure

Resolution	35.396 - 2.100
R-factor	0.2146
Rwork	0.213
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7i
RMSD bond length	0.010
RMSD bond angle	1.211
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	46284
<I/σ(I)>	9.39
Completeness [%]	99.4
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	Ammonium sulfate, PEG8000