6JBE

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.990, 84.990, 145.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.430 - 1.750
R-factor	0.1407
Rwork	0.139
R-free	0.17570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.018
RMSD bond angle	1.770
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.430	9.090
High resolution limit [Å]	1.750	1.750
Rmerge	0.092	0.606
Rmeas	0.099	0.652
Rpim	0.037	0.238
Number of reflections	52985	2590
<I/σ(I)>	17.4	3.9
Completeness [%]	97.0	88.3
Redundancy	12.2	12.7
CC(1/2)	0.999	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350