6JB8

Crystal structure of nanobody D3-L11 in complex with hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-01
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	40.240, 49.480, 59.280
Unit cell angles	90.00, 93.40, 90.00

Refinement procedure

Resolution	40.170 - 1.650
R-factor	0.17027
Rwork	0.169
R-free	0.20691
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jb2
RMSD bond length	0.019
RMSD bond angle	1.815
Data reduction software	MOSFLM (7.1.0)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.170	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.076	0.399
Number of reflections	26392	2783
<I/σ(I)>	11.1	2.2
Completeness [%]	93.7	68
Redundancy	3.5	2.8
CC(1/2)	0.997	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	20mM Tris-HCl, 100mM NaCl, 100mM NaNO3, 16% PEG 3350