6JB6

Crystal structure of Ub-conjugated Ube2K C92K&K97A mutant (isopeptide linkage), 2.7 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	95
Detector technology	CCD
Collection date	2015-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	145.798, 38.200, 56.180
Unit cell angles	90.00, 93.55, 90.00

Refinement procedure

Resolution	45.810 - 2.700
R-factor	0.2477
Rwork	0.244
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.712
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.670	2.670
Number of reflections	9091
<I/σ(I)>	8.9
Completeness [%]	99.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, PEG 400, magnesium chloride