6JB2
Crystal structure of nanobody D3-L11 mutant Y102A in complex with hen egg-white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.040, 49.400, 58.020 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 33.970 - 1.500 |
| R-factor | 0.12608 |
| Rwork | 0.125 |
| R-free | 0.16494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zvy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.575 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.970 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.480 |
| Number of reflections | 39075 | 4911 |
| <I/σ(I)> | 11.9 | 2.8 |
| Completeness [%] | 97.8 | 85 |
| Redundancy | 5.3 | 3.9 |
| CC(1/2) | 0.996 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 20mM TRIS HCl, 100mM NaCl, 100mM Sodium nitrate, 16% (w/v) PEG 3350 |
