6JAR
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R49A in complex with maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-03-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.980, 84.980, 145.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.380 - 1.630 |
| R-factor | 0.1412 |
| Rwork | 0.140 |
| R-free | 0.16620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9w |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.932 |
| Data reduction software | iMOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.380 | 8.920 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.064 | 0.391 |
| Rmeas | 0.068 | 0.416 |
| Rpim | 0.022 | 0.139 |
| Number of reflections | 61325 | 2721 |
| <I/σ(I)> | 24.3 | 5.1 |
| Completeness [%] | 91.3 | 83.4 |
| Redundancy | 16.4 | 16.3 |
| CC(1/2) | 0.999 | 0.954 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.4 | 277 | 0.04 M Citric Acid, 0.06 Bis-Tris Propane, 20% PEG 3350 |
