6JAR

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R49A in complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	84.980, 84.980, 145.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.380 - 1.630
R-factor	0.1412
Rwork	0.140
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.020
RMSD bond angle	1.932
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.380	8.920
High resolution limit [Å]	1.630	1.630
Rmerge	0.064	0.391
Rmeas	0.068	0.416
Rpim	0.022	0.139
Number of reflections	61325	2721
<I/σ(I)>	24.3	5.1
Completeness [%]	91.3	83.4
Redundancy	16.4	16.3
CC(1/2)	0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 Bis-Tris Propane, 20% PEG 3350