6JAP

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in complex with sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-03-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	85.410, 85.410, 146.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.760 - 1.770
R-factor	0.1526
Rwork	0.150
R-free	0.19530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j9w
RMSD bond length	0.018
RMSD bond angle	1.799
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.760	9.040
High resolution limit [Å]	1.770	1.770
Rmerge	0.063	0.247
Rmeas	0.067	0.263
Rpim	0.022	0.090
Number of reflections	53038	2950
<I/σ(I)>	31.2	8.8
Completeness [%]	99.7	98.6
Redundancy	17.2	15.9
CC(1/2)	0.999	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.4	277	0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350