6J9X
Crystal structure of Trypanosoma brucei gambiense glycerol kinase phosphorylated at Thr12(pyrophosphatase reaction)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.380, 119.340, 154.730 |
Unit cell angles | 90.00, 90.82, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.700 |
R-factor | 0.18688 |
Rwork | 0.184 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wxl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.589 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.050 | 0.770 |
Number of reflections | 61873 | 18869 |
<I/σ(I)> | 8.25 | 0.8 |
Completeness [%] | 95.9 | 95.3 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL |